ABSTRACT
High fat diet fed rats showed significant increased levels of plasma and tissue total cholesterol, triglycerides, free fatty acids, phospholipids, plasma LDL cholesterol and decreased level of plasma HDL cholesterol. Methanolic extract of D. biflorus administration to high fat diet fed rats showed near to normal levels of the above lipids in plasma and tissues. Higher dose of the extract (400 mg/kg body weight) showed comparable results with standard drug atorvastatin. It is concluded that the methanolic extract of D. biflorus possesses hypolipidemic activity in high fat diet fed rats.
Subject(s)
Animals , Cholesterol/metabolism , Cholesterol, HDL/metabolism , Cholesterol, LDL/metabolism , Diet, Atherogenic , Dietary Fats/metabolism , Dolichos/metabolism , Fatty Acids/metabolism , Heptanoic Acids/pharmacology , Lipid Metabolism , Lipids/chemistry , Lipoproteins/chemistry , Male , Methanol/chemistry , Phospholipids/metabolism , Plant Extracts/pharmacology , Pyrroles/pharmacology , Rats , Rats, Wistar , Triglycerides/chemistryABSTRACT
Rats fed excess vitamin A showed decreased body weight gain and protein efficiency ratio. In rats fed low protein vitamin A level increased in liver but with an associated decrease in plasma. These changes were reversed in high protein fed state. The amount of protein in diet had little effect on haemoglobin level in erythrocyte, but excess vitamin A in diet significantly decreased haemoglobin level in erythrocyte. Lipid peroxidation (LP) increased in rats fed low protein and decreased in high protein fed rats. Rats fed high protein and excess vitamin A showed minimum level of LP. Result showed that high protein in diet increased the levels of antioxidant enzymes, catalase and superoxide dismutase (SOD) and that excess vitamin A supplementation functions synergistically with high protein in diet to increase antioxidant enzymes level.
Subject(s)
Animals , Catalase/metabolism , Dietary Proteins/administration & dosage , Erythrocytes/metabolism , Glutathione Peroxidase/metabolism , Hemoglobins/analysis , Hypervitaminosis A/metabolism , Lipid Peroxidation , Lipid Peroxides/metabolism , Liver/drug effects , Male , Oxidoreductases/metabolism , Protein Deficiency , Rats , Rats, Wistar , Superoxide Dismutase/metabolism , Vitamin A/bloodABSTRACT
Level of vitamin A increased in plasma and liver in hypervitaminotic A albino rats fed normal quantity of protein in diet. In low protein fed state vitamin A level in liver increased due to accumulation of vitamin A and lack of carrier protein with an associated decrease of plasma vitamin A. In high protein fed rats the level of vitamin A in plasma increased due to enhanced transport while in liver it decreased. The results indicate that for normal transport of vitamin A adequate plasma protein level is essential.